human heart tissue (Novus Biologicals)
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Novus Biologicals
human heart tissue
Human Heart Tissue, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heart+tissue+lysate/Human+Heart+Whole+Tissue+Lysate+(Adult+Whole+Congenital+Heart)/pmc13017279-158-0-8
Average 94 stars, based on 3 article reviews
Human Heart Tissue, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heart+tissue+lysate/Human+Heart+Whole+Tissue+Lysate+(Adult+Whole+Congenital+Heart)/pmc13017279-158-0-8
Average 94 stars, based on 3 article reviews
human heart tissue - by Bioz Stars,
2026-11
94/100 stars
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SDS Page:Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity. Article Snippet: C4BP competition with M2G antiserum ELISAs were carried out as aforementioned, except that M2G antiserum (103 dilution) in PBS was coated in the wells of 96-well microtiter plates overnight at 4 C. His6-tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or Article Title: Design of M protein immunogens to elicit broadly reactive antibodies against Streptococcus pyogenes Article Snippet: Wells were blocked with 1% BSA in TBST for 1 h, and then incubated with 10 μg/mL of His6-tagged M proteins (N-terminal 100 amino acids, diluted in 1% BSA/ TBST) for 1.5 h. To detect bound M proteins, 100 μl HRP- conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/ TBST, BioLegend) was added to the wells and incubated for 1 h. .. Twenty μg of normal adult human brain tissue lysate (Novus Biologicals) or Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity Article Snippet: His 6 -tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or Membrane:Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity. Article Snippet: C4BP competition with M2G antiserum ELISAs were carried out as aforementioned, except that M2G antiserum (103 dilution) in PBS was coated in the wells of 96-well microtiter plates overnight at 4 C. His6-tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or Article Title: Design of M protein immunogens to elicit broadly reactive antibodies against Streptococcus pyogenes Article Snippet: Wells were blocked with 1% BSA in TBST for 1 h, and then incubated with 10 μg/mL of His6-tagged M proteins (N-terminal 100 amino acids, diluted in 1% BSA/ TBST) for 1.5 h. To detect bound M proteins, 100 μl HRP- conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/ TBST, BioLegend) was added to the wells and incubated for 1 h. .. Twenty μg of normal adult human brain tissue lysate (Novus Biologicals) or Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity Article Snippet: His 6 -tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or Western Blot:Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity. Article Snippet: C4BP competition with M2G antiserum ELISAs were carried out as aforementioned, except that M2G antiserum (103 dilution) in PBS was coated in the wells of 96-well microtiter plates overnight at 4 C. His6-tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or Article Title: Design of M protein immunogens to elicit broadly reactive antibodies against Streptococcus pyogenes Article Snippet: Wells were blocked with 1% BSA in TBST for 1 h, and then incubated with 10 μg/mL of His6-tagged M proteins (N-terminal 100 amino acids, diluted in 1% BSA/ TBST) for 1.5 h. To detect bound M proteins, 100 μl HRP- conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/ TBST, BioLegend) was added to the wells and incubated for 1 h. .. Twenty μg of normal adult human brain tissue lysate (Novus Biologicals) or Article Title: A conserved 3D pattern in a Streptococcus pyogenes M protein immunogen elicits M-type crossreactivity Article Snippet: His 6 -tagged M protein constructs (0.5 μM) were added alone or with 0.7 μM C4BP or 5 μM fibrinogen (EMD Millipore), with which they had first been incubated for 10 min, to wells and incubated for 1.5 h. Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST). .. Twenty micrograms of normal adult HB tissue lysate (Novus Biologicals) or |